Journal: Human Vaccines & Immunotherapeutics
Article Title: Unexpectedly competent immune response to SARS-CoV-2 vaccination in Rett syndrome
doi: 10.1080/21645515.2026.2612817
Figure Lengend Snippet: Spike-specific immune responses following SARS-CoV-2 vaccination in RTT and HC. (A) Longitudinal spike-specific IgG analysis from baseline (0) to 6 months post third dose (day 450) in HC with overlaid single values assessed in RTT patients. The first (v1, day 0), second (v2, day21) and third (v3, day270) vaccine dose administration is shown by arrows. (B) Spike-specific IgG titres (GMT with range) detected in RTT patients vaccinated with two (v2) or three (v3) doses, with (+) or without (–) SARS-CoV-2 infection. (C) ACE2/RBD binding inhibition assay. The dotted line at 30% inhibition percentage, indicates the threshold of positivity of the assay. (D) RBD + MBCs gating strategy and (E) frequencies of RBD + MBC in RTT and HC following two and three vaccine doses in SARS-CoV-2 infected (filled symbols) or non-infected (open symbols) individuals. (F) FlowSOM clustering analysis performed on total MBCs (left heatmap), and RBD + MBCs (right heatmap). (G) Spike-specific IgG-secreting MBCs assessed by ELISpot assay and (H) their frequencies in RTT and HC. Data from SARS-CoV-2 infected individuals are shown as filled symbols. Statistical differences were assessed by Mann–Whitney test.
Article Snippet: Recombinant SARS-CoV-2 full Spike protein (Sino Biological; 1 ug/ml protein) was used for coating wells in ELISA assay.
Techniques: Infection, Binding Assay, Inhibition, Enzyme-linked Immunospot, MANN-WHITNEY